Hepatocyte Transplantation Methods and Protocols

Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel...

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Bibliographic Details
Other Authors: Dhawan, Anil (Editor), Hughes, Robin D. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2009, 2009
Edition:1st ed. 2009
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Human Hepatocyte Transplantation Overview
  • Isolation of Human Hepatocytes
  • An Optimised Method for Cryopreservation of Human Hepatocytes
  • Liver Cell Culture Techniques
  • In vitro Assays for Induction of Drug Metabolism
  • Hepatocyte Apoptosis
  • Small Animal Models of Hepatocyte Transplantation
  • Hepatocyte Transplantation Techniques: Large Animal Models
  • Cell Transplant Techniques: Engraftment Detection of Cells
  • Hepatic Preconditioning for Transplanted Cell Engraftment and Proliferation
  • Ex vivo Gene Transfer into Hepatocytes
  • Sources of Adult Hepatic Stem Cells: Haematopoietic
  • Production of Hepatocyte-like Cells from Human Amnion
  • Generation of Hepatocytes from Human Embryonic Stem Cells
  • Isolation, In vitro Cultivation and Characterisation of Foetal Liver Cells
  • Human Intrahepatic Biliary Epithelial Cell Lineages: Studies in vitro
  • Liver Cell Labelling with MRI Contrast Agents
  • Microbiological Monitoring of Hepatocyte Isolation in the GMP Laboratory