Virus-Host Interactions Methods and Protocols

Virus-Host Interactions: Methods and Protocols covers various aspects of virological research, such as biochemical approaches, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level with a strong focus on the manifold possibilities to study protein-protein...

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Bibliographic Details
Other Authors: Bailer, Susanne M. (Editor), Lieber, Diana (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2013, 2013
Edition:1st ed. 2013
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes
  • Determination of HSV-1 Infectivity Using a Luciferase Reporter Cell Line
  • Generation of a Cell Line for Constitutive Expression of Host or Viral miRNAs
  • Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry
  • High Temporal Resolution Imaging Reveals Endosomal Membrane Penetration and Escape of Adenoviruses in Real-Time
  • Three-Dimensional Visualization of Virus Infected Cells by Serial Sectioning - An Electron Microscopic Study Using Resin Embedded Cells
  • 3D Tissue Model for Herpes Simplex Virus-1Infections
  • In vivo Visualization of Encephalitic Lesions in Herpes Simplex Virus Type 1 (HSV-1) Infected Mice by Magnetic Resonance Imaging (MRI)
  • Detection of Antigen-Specific T Cells Based on Intracellular Cytokine Staining Using Flow-Cytometry
  • Functional NK Cell Cytotoxicity Assays Against Virus Infected Cells
  • Assessment of Natural Killer Cell Responses to Human Cytomegalovirus Infected Macrophages
  • Analysis of Cell Migration During HCMV Infection
  • Gene Targeting in Mice: A Review
  • A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant ES Cells
  • Measurement of Mouse Cytomegalovirus Induced Interferon-β with Immortalized Luciferase Reporter Cells
  • A High-Throughput Yeast Two Hybrid Protocol to Determine Virus-Host Protein Interactions
  • Analysis of Protein-Protein Interactions Using LUMIER Assay
  • Detection of Protein Interactions During Virus Infection by Bimolecular Fluorescence Complementation
  • Discovery of Host-Viral Protein Complexes During Infection
  • Screening for Host Proteins with Pro- and Antiviral Activity Using High-Throughput RNAi
  • High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay by Metabolic Labelling of Newly Transcribed RNA using 4-thiouridine
  • Isolation and Characterization of Pathogen-Bearing Endosomes Enables Identification of New Cellular Cofactors and Analysis of Endosomal Escape
  • Computational Analysis of Virus-Host Interactomes
  • Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins
  • Detection of Integrated Herpesvirus Genomes by Fluorescent in situ Hybridization (FISH)