Adhesion Protein Protocols

Cellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourige...

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Bibliographic Details
Other Authors: Coutts, Amanda S. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2013, 2013
Edition:3rd ed. 2013
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Reconstructing Integrin Activation in vitro
  • Quantifying Cellular Adhesion to Covalently Immobilized Extracellular Matrix Proteins by Single-Force Spectroscopy
  • A Multimode-TIRFM & Microfluidic Technique to Examine Platelet Adhesion Dynamics
  • Localization-Based Super-Resolution Imaging of Cellular Structures
  • Use of Microarray Analysis to Investigate EMT Gene Signatures.-  Podosome Reformation in Macrophages: Assays and Analysis
  • Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells
  • Basement Membrane Invasion Assays: Native Basement Membrane and Chemoinvasion Assay
  • Cytoplasmic Actin: Purification and Single Molecule Assembly Assays
  • Synthetic and Tissue-Derived Models for Studying Rigidity Effects on Invadopodia Activity
  • Determining Rho GTPase Activity by an Affinity-Precipitation Assay
  • Proteomic and Biochemical Methods to Study the Cytoskeletome. Fabricating Surfaces with Distinct Geometries and Different Combinations of Cell Adhesion Proteins
  • Purification of Native Arp2/3 Complex from Bovine Thymus
  • Purification of Arp2/3 Complex from Saccharomyces Cerevisiae
  • Measurement and Analysis of in vitro Actin Polymerization
  • Use of the xCELLigence System for Real Time Analysis of changes in Cellular Motility and Adhesion in Physiological Conditions
  • Measuring Chemotaxis Using Direct Visualisation Microscope Chambers
  • In Vitro Microtubule Severing Assays
  • VE-Cadherin Status asan Indicator of Microvascular Permeability
  • High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis
  • Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells
  • Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion
  • Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions
  • VE-Cadherin Status as an Indicator of Microvascular Permeability
  • High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis. Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells
  • Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion
  • Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions