The Inflammasome Methods and Protocols
Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details o...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2013, 2013
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| Edition: | 1st ed. 2013 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Assessing b-amyloid-induced NLRP3 Inflammasome Activation in Primary Microglia
- Activating the NLRP3 Inflammasome using the Amyloidogenic Peptide IAPP
- Assessment and Quantification of Crystal-induced Lysosomal Damage
- Assessment of Inflammasome Activation in Primary Human Immune Cells
- NLRP3 Inflammasome Activation and Cytotoxicity Induced by particulate Adjuvants
- Measuring Inflammasome Activation in Response to Bacterial Infection
- Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity
- ASC Speck Formation as a Read-out for Inflammasome Activation
- Immunoblotting for Active Caspase-1
- Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1α, IL-1β and Caspase-1
- Reconstituting the NLRP1 Inflammasome in vitro
- Assessing ATP Binding and Hydrolysis by NLR Proteins
- Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition
- Analysis of Microbiota Alterations in Inflammasome-deficient Mice
- Quantification of Adipose Tissue Leukocytosis in Obesity
- In vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates