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Pluripotent Stem Cells Methods and Protocols

Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are deriv...

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Bibliographic Details
Other Authors: Lakshmipathy, Uma (Editor), Vemuri, Mohan C. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2013, 2013
Edition:1st ed. 2013
Series:Methods in Molecular Biology
Subjects:
Cell Biology
Cytology
Stem Cells
Stem Cell Biology
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Sources and Derivation of Human Embryonic Stem Cells
  • A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells
  • A Review of the Methods for Human iPSC Derivation
  • Generation of Human Induced Pluripotent Stem Cells by Lentiviral Transduction
  • Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus
  • Generation of Human Induced Pluripotent Stem Cells (hiPSCs) using Episomal Vectors on Defined Essential 8TM Medium Conditions
  • Feeder-free Substrates for Pluripotent Stem Cell Culture
  • Methods for Culturing Human Embryonic Stem Cells on Feeders
  • Methods for Culturing Human Embryonic Stem Cells in a Xeno-Free System
  • Directed Differentiation of Human Pluripotent Stem Cells Along the Pancreatic Endocrine Lineage
  • Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes
  • Highly Efficient Directed Differentiation of Human Induced Pluripotent Stem Cells into Cardiomyocytes
  • Generation of iPSC Lines from Human Normal and Neoplastic Blood Cells Using Episomal Vectors
  • Cellular Characterization of Human Pluripotent Stem Cells
  • TaqMan® OpenArray® High Throughput Transcriptional Analysis of Human Embryonic and Induced Pluripotent Stem Cells
  • Epigenome Analysis of Pluripotent Stem Cells
  • Single Cell Gene Expression Analysis of Pluripotent Stem Cells
  • Profiling Stem Cells Using Quantitative PCR Protein Assays
  • Labeled Stem Cells as Disease Models and in DrugDiscovery
  • Cloning Technologies
  • Stable Transfection Using Episomal Vectors to Create Modified Human Embryonic Stem Cells
  • Site-Specific Integration in Human ESC Using Jump-In™ TI™ Technology

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