Transgenic Cotton Methods and Protocols

Cotton is the most important textile and cash crop and is widely cultivated in more than 70 countries, including the United States, China, and India. Because of its long life cycle and complicated genetic background, it is hard to improve cotton using traditional breeding techniques although it has...

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Bibliographic Details
Other Authors: Zhang, Baohong (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2013, 2013
Edition:1st ed. 2013
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Transgenic Cotton: From Biotransformation Methods to Agricultural Application
  • Genetically Modified Cotton in India and Detection Strategies
  • Agrobacterium-Mediated Transformation of Cotton
  • Biolistic Transformation of Cotton Zygotic Embryo Meristem
  • Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures
  • Cotton Transformation via Pollen Tube Pathway
  • Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.)
  • Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome
  • Estimating the Copy Number of Transgenes in Transformed Cotton by Real-Time Quantitative PCR
  • Development of Enzyme-Linked Immunosorbant (ELISA) Assay for the Detection of Bt Protein in Transgenic Cotton
  • DNA-Based Diagnostics for Genetically Modified Cotton: Decaplex PCR Assay to Differentiate MON531 and MON15985 Bt Cotton Events
  • A Simple and Rapid Method for Determining Transgenic Cotton Plants
  • An Efficient Grafting Technique for Recovery of Transgenic Cotton Plants
  • Inheritance of Transgenes in Transgenic Bt Lines Resistance to Helicoerpa armigera in Upland Cotton
  • Agrobacterium rhizogenes-Induced Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics
  • Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation
  • Development of Transgenic CryIA(c)+GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover
  • Agrobacterium-Mediated Transformation of Cotton (Gossypium hirsutum) Shoot Apex with a Fungal Phytase Gene Improves Phosphorus Acquisition
  • Genetic Transformation of Cotton with a Harpin-Encoding Gene hpaXoo Confers an Enhanced Defense Response Against Verticillium dahliae Kleb
  • Development of Insect-Resistant Transgenic Cotton with Chimeric TVip3A* Accumulating in Chloroplasts
  • Determining Gene Flow in Transgenic Cotton