Legionella Methods and Protocols
Legionnaires’ disease is a severe form of pneumonia that can occur in epidemics of several hundred cases. This atypical pneumonia is characterized by a high mortality rate and affects primarily elderly and immuno-compromised individuals. An in-depth understanding of the ecology and virulence of Legi...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2013, 2013
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| Edition: | 1st ed. 2013 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Identification of Legionella in the Environment
- Identification of Legionella in Clinical Samples
- Sequence-Based Identification of Legionella
- Investigation of Outbreaks - Epidemiology
- Outbreak Investigations and Identification of Legionella in Contaminated Water
- Typing Methods for Legionella
- Culture, Media and Handling of Legionella
- The Intracellular Metabolism of Legionella by Isotopologue Profiling
- Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction
- Constructing Unmarked Gene Deletions in Legionella pneumophila
- Natural Biofilm Formation with Legionella pneumophila
- Production of Legionella pneumophila Monospecies Biofilms in a Low-Nutrient Concentration Medium
- Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila
- Analysis of Legionella Infection by Flow Cytometry
- Analysis of Legionella Infection Using RNAi in Drosophila Cells
- Analyzing Caspase-1 Activation During Legionella pneumophila Infection in Macrophages
- The Mouse as a Model for Pulmonary Legionella Infection
- Assessment of Legionella-Specific Immunity in Mice
- The Guinea Pig Model of Legionnaires’ Disease
- Human Susceptibility to Legionnaires´ Disease
- cDNA library Construction for Next Generation Sequencing (NGS) to Determine the Transcriptional Landscape of Legionella pneumophila
- Methods to Study Legionella Transcriptome in vitro and in vivo
- Co-Immunoprecipitation: Protein-RNA and Protein-DNA Interaction
- Identification of Legionella Effectors UsingBioinformatic Approaches
- Immunofluorescence Imaging of Legionella Infection In vitro
- Electron Microscopy of Legionella and Legionella-Infected Cells
- Pathogen Vacuole Purification from Legionella-Infected Amoeba and Macrophages
- Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System
- Subcellular Localization of Legionella Dot/Icm Effectors
- Purification and Characterization of Legionella U-Box-Type E3 ubiquitin ligase
- Legionella Phospholipases Implicated in Infection – Determination of Enzymatic Activities
- Interactions of Legionella Effector Proteins with Host Phosphoinositide Lipids
- Characterization of Legionella lipopolysaccharide
- The Tetrahymena and Acanthamoeba Model Systems
- Dictyostelium Host Response to Legionella infection: Strategies and Assays
- The Caenorhabditis elegans Model of Legionella Infection
- Infection of Nonphagocytic Host Cells by Legionella