Single-Stranded DNA Binding Proteins : Methods and Protocols

The genomes of cellular organisms are organized as double-stranded DNA, a structure that must be unwound to provide DNA replication, recombination, and repair machinery access to genomic information.  However, DNA unwinding comes with inherent risks to genome stability.  To help mediate these risks,...

Full description

Corporate Author: SpringerLink (Online service)
Other Authors: Keck, James L. (Editor)
Format: eBook
Published: Totowa, NJ Humana Press 2012, 2012
Edition:1st ed. 2012
Series:Methods in Molecular Biology
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Functions of Single-Strand DNA-Binding Proteins in DNA Replication, Recombination and Repair.-Structural Diversity Based on Variability in Quaternary Association. A Case Study Involving Eubacterial and Related SSBs
  • SB Binding to ssDNA Using Isothermal Titration Calorimetry
  • SSB-DNA Binding Monitored by Fluorescence Intensity and Anisotropy
  • Single Molecule Analysis of SSB Dynamics on Single Stranded DNA
  • Sample Preparation Methods to Analyze DNA-induced Structural Changes in Replication Protein A (RPA)
  • Structural Studies of SSB Interaction with RecO
  • Investigation of Protein-Protein Interactions of Single-Stranded DNA-Binding Proteins by Analytical Ultracentrifugation
  • Ammonium Sulfate Co-Precipitation of SSB and Interacting Proteins
  • Analyzing Interactions Between SSB and Proteins by the Use of Fluorescence Anisotropy
  • Far Western Blotting as a Rapid and Efficient Method for Detecting Interactions Between DNA Replication and DNA Repair Proteins
  • Methods for Analysis of SSB/Protein Interactions by SPR
  • Use of Native Gels to Measure Protein Binding to SSB
  • Identification of Small Molecules That Disrupt SSB/Protein Interactions Using a High-Throughput Screen
  • Detection of Post-translational Modifications of Replication Protein A
  • Detecting Posttranslational Modification of Bacterial SSB Proteins
  • Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA
  • Fluorescent Single-Stranded DNA-Binding Proteins Enable in vitro and in vivo Studies<
  • Use of Fluorescently Tagged SSB Proteins in in vivo Localization Experiments