Difference Gel Electrophoresis (DIGE) Methods and Protocols
Divided into four concise sections, this detailed volume opens with the basics of DIGE, the technique and its practical details with a focus on the planning of a DIGE experiment and its data analysis. The next section introduces various DIGE methods from those employed by scientists world-wide to mo...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2012, 2012
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| Edition: | 1st ed. 2012 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- DIGE: Past and Future
- The Basics of 2D DIGE
- Multifluorescence 2D Gel Imaging and Image Analysis
- Assessing Signal-to-Noise in Quantitative Proteomics: Multivariate Statistical Analysis in DIGE Experiments
- Analysis of Proteins Using DIGE and MALDI Mass Spectrometry
- Synthesis and Validation of Cyanin-Based Dyes for DIGE
- 2D DIGE Saturation Labeling for Minute Sample Amounts
- Proteomic Analysis of Redox-Dependent Changes Using Cysteine-Labeling 2D DIGE
- Analysis of Protein Post-Translational Modifications Using DIGE-Based Proteomics
- Comparative Analyses of Protein Complexes by Blue Native DIGE
- 2D DIGE Analysis of Protein Extracts from Muscle Tissue
- Combination of Highly Efficient Hexapeptide Ligand Library-Based Sample Preparation with 2D DIGE for the Analysis of the Hidden Human Serum/Plasma Proteome
- 2D DIGE Analysis of Serum after Fractionation by ProteoMinerTM Beads
- Study Design in DIGE-Based Biomarker Discovery
- Comparative 2D DIGE Analysis of the Depleted Serum Proteome for Biomarker Discovery
- Differential Gel-Based Proteomic Approach for Cancer Biomarker Discovery Using Human Plasma
- 2D DIGE for the Analysis of RAMOS Cells Sub-Proteomes
- Application of Saturation Labeling in Lung Cancer Proteomics
- Proteomic Profiling of the Epithelial-Mesenchymal Transition Using 2D DIGE
- Method for Protein Subfractionation of Cardiovascular Tissues before DIGE Analysis.-Application of DIGE and Mass Spectrometry in the Study of Type 2 Diabetes Mellitus (T2DM) Mouse Models
- Evaluating the Efficacy of Subcellular Fractionation of Blast Cells Using Live Cell Labeling and 2D DIGE
- DIGE Analysis of Plant Tissue Proteomes Using a Phenolic Protein Extraction Method
- Native DIGE of Fluorescent Plant Protein Complexes
- An Overview of 2D DIGE Analysis of Marine (Environmental) Bacteria
- Application of 2D DIGE in Animal Proteomics