Handbook of ELISPOT Methods and Protocols
Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccin...
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2012, 2012
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| Edition: | 2nd ed. 2012 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Unique Strengths of ELISPOT for T Cell Diagnostics
- The Impact of Harmonization on ELISPOT Assay Performance
- Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells
- Utilization of Feline ELISPOT for Mapping Vaccine Epitopes
- Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay
- Dual and Triple Color Fluorospot
- ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes
- ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells
- ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion
- Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell
- How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells’ Secretory Activity
- Mathematical Algorithms for Automatic Search, Recognition, and Detection of Spots in ELISPOT Assay
- Objective, User-Independent ELISPOT Data Analysis Based on Scientifically Validated Principles
- Statistical Analysis of ELISPOT Assays
- Response Determination Criteria for ELISPOT: Toward a Standard that Can Be Applied Across Laboratories
- Detection of Vaccinia Virus-Specific IFNγ and IL-10 Secretion from Human PBMCs and CD8+ T Cells by ELISPOT
- ELISPOT Assays to Enumerate Bovine IFN-γ Secreting Cells for the Development of Novel Vaccines Against Bovine Tuberculosis
- IL-7 Addition Increases Spot Size and Number as Measured by T-SPOT.TB®
- Overview of Membranes and Membrane Plates Used in Research and Diagnostic ELISPOT Assays