Platelets and Megakaryocytes Volume 3, Additional Protocols and Perspectives

New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established. Thus, the main aim of Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives is to complement the first two vo...

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Bibliographic Details
Other Authors: Gibbins, Jonathan M. (Editor), Mahaut-Smith, Martyn P. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2012, 2012
Edition:1st ed. 2012
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Intravital Imaging of Thrombus Formation in Small and Large Mouse Arteries: Experimentally-Induced Vascular Damage and Plaque Rupture In Vivo
  • Assessment of Platelet Aggregation Responses In Vivo in the Mouse
  • Focal Cerebral Ischemia
  • Extracorporeal Assays of Thrombosis
  • Platelet Life Span and Apoptosis
  • A Live Cell Micro-Imaging Technique to Examine Platelet Calcium Signalling Dynamics Under Blood Flow
  • Platelet Shape Change and Spreading
  • Clot Retraction
  • Visualization and Manipulation of the Platelet and Megakaryocyte Cytoskeleton
  • Measurement of Platelet Microparticles
  • Assessing Protein Synthesis by Platelets
  • A Rapid and Efficient Platelet Purification Protocol for Platelet Gene Expression Studies
  • Characterization of Megakaryocyte Development in the Native Bone Marrow Environment
  • Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow
  • In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
  • Megakaryocyte and Platelet Production from Human Cord Blood Stem Cells
  • Culture of Megakaryocytes and Platelets from Subcutaneous Adipose Tissue and a Preadipocyte Cell Line
  • Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression
  • Assessment of Megakaryocyte Migration and Chemotaxis
  • Megakaryopoiesis and Thrombopoiesis: An Update on Cytokines and Lineage Surface Markers
  • Using Zebrafish (Danio rerio) to Assess Gene Function in Thrombus Formation
  • Platelet Receptor Shedding
  • Endogenous Inhibitory Mechanisms and the Regulation of Platelet Function
  • Platelet Proteomics: State of the Art and Future Perspective