MicroRNAs in Development Methods and Protocols
Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2011, 2011
|
| Edition: | 1st ed. 2011 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- In situ Detection of microRNAs in Animals
- Detection of microRNAs in Plants by In situ Hybridization
- Detecting Animal and Plant microRNAs by Northern Blotting
- Profiling microRNAs by Real-time PCR
- Detection of Small RNAs and microRNAs using Deep Sequencing Technology
- Detection of microRNAs in Prostate Cancer Cells by microRNA Array
- microRNA Knock Down by Cholesterol Conjugated Antisense Oligos in Mouse Organ Culture
- Protocols for Use of Homologous Recombination Gene-targeting to Produce microRNA Mutants in Drosophila
- Engineering Elements for Gene Silencing: The Artificial microRNAs Technology
- Mimicry Technology: Suppressing Small RNA Activity in Plants
- Experimental Validation of MicroRNA Targets using a Luciferase Reporter System
- Experimental Identification of microRNA Targets by Immunoprecipitation of Argonaute Protein Complexes
- Comprehensive Identification of miRNA Target Sites in Live Animals
- Target Validation of Plant microRNAs.- A High-throughput Sequencing-based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes