Serum/Plasma Proteomics Methods and Protocols
Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood pro...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2011, 2011
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| Edition: | 1st ed. 2011 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Methods for Quantitative Assaying of Blood-Based Biomarkers. Biomarker Validation in Blood Specimens by Selected Reaction Monitoring Mass Spectrometry of N-glycosites. A Fluorescent Microsphere-based Method for Assay of Multiple Analytes in Plasma. Immuno-mass Spectrometry: Quantification of Low Abundance Proteins in Biological Fluids. Qualitative and Quantitative Analysis of Glycated Proteins in Human Plasma by Glucose Isotopic Labeling with 13C6-reducing Sugars
- Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids. Exosome Isolation for Proteomic Analyses and RNA Profiling. Extraction and Proteome Analysis of Liver Tissue Interstitial Fluid. A Protocol for the Preparation of Cryoprecipitate and Cryo-depleted Plasma
- Preparation of Platelet Concentrates. Phosphoproteome Analysis of the Platelet Plasma Membrane
- Bioinformatic Analysis of Blood Proteins and Peptides.Statistical Design and Analysis of Label-free LC-MS Proteomic Experiments: A Case Study of Coronary Artery Disease. Data Management in Mass Spectrometry-based Proteomics. Bioinformatics Challenges in the Proteomic Analysis of Human Plasma. Using the Human Plasma PeptideAtlas to Study Human Plasma Proteins
- Fractionation Strategies for In-depth Blood Proteome Analysis. Plasma Biomarker Discovery using 3-D Protein Profiling Coupled with Label-free Quantitation. Intact Protein Separation by 1 and 2-dimension Liquid Chromatography for Comparative Proteomic Separation of Partitioned Serum or Plasma. In-depth Analysis of a Plasma or Serum Proteome using a 4-D Protein Profiling Method. Intact-protein Analysis System (IPAS) for Discovery of Serum-based Disease Biomarkers. Model-based Discovery of Circulating Biomarkers. Low-molecular Weight Plasma Proteome Analysis using Centrifugal Ultrafiltration. High-throughput Analysis of Glycoproteins from Plasma
- Blood Collection and Handling Strategies. Minimizing Pre-analytical Variation of Plasma Samples by Proper Blood Collection and Handling. Collection and Handling of Blood Specimens for Peptidomics. Investigation of Peptide Biomarker Stability in Plasma Samples using Time-course MS Analysis