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130626 ||| eng |
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|a 9781617790126
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| 100 |
1 |
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|a Satyanarayanajois, Seetharama D.
|e [editor]
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| 245 |
0 |
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|a Drug Design and Discovery
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Seetharama D. Satyanarayanajois
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| 250 |
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|a 1st ed. 2011
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 2011, 2011
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| 300 |
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|a XI, 284 p
|b online resource
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| 505 |
0 |
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|a Virtual Screening for Lead Discovery -- Computer-Aided Drug Discovery and Development -- Using Active Site Mapping and Receptor-Based Pharmacophore Tools: Prelude to Docking and De Novo/Fragment-Based Ligand Design -- Methods for Evaluation of Structural and Biological Properties of Anti-Invasive Natural Products -- Solid-Phase Peptide Synthesis Using Microwave Irradiation -- Fluorescent and Lanthanide Labeling for Ligand Screens, Assays, and Imaging -- DNA-Directed Assembly Microarray for Protein and Small Molecule Inhibitor Screening -- Selection of Peptide Ligands for Human Placental Transcytosis Systems Using In vitro Phage Display -- Optimization of the Tetrazolium Dye (MTT) Colorimetric Assay for Cellular Growth and Viability -- Imaging NF- κB Signaling in Mice for Screening Anti-Cancer Drugs -- Evaluation of Anticancer Agents Using Flow Cytometry Analysis of Cancer Stem Cells -- Chemical Screening with Zebrafish Embryos.- iTRAQTM Labeling Coupled with LC-MALDI Mass Spectrometry for Monitoring Temporal Response of Colorectal Cancer Cells to Butyrate Treatment -- Heterotypic Cell Adhesion Assay for the Study of Cell Adhesion Inhibition -- Evaluation of Antibacterial Activity of Proteins and Peptides Using a Specific Animal Model for Wound Healing -- In vitro Immunogenicity Risk Assessment of Therapeutic Proteins in Preclinical Setting
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| 653 |
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|a Pharmacology
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| 653 |
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|a Laboratory Medicine
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| 653 |
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|a Laboratory medicine
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| 653 |
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|a Pharmacology/Toxicology
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b Springer
|a Springer eBooks 2005-
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| 490 |
0 |
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|a Methods in Molecular Biology
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-61779-012-6?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 615
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| 520 |
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|a Research in the pharmaceutical sciences and medicinal chemistry has taken an important new direction in the past two decades with a focus on large molecules, especially peptides and proteins, as well as DNA therapeutics. In Drug Design and Discovery: Methods and Protocols, leading experts provide an in-depth view of key protocols that are commonly used in drug discovery laboratories. Covering both classic and cutting-edge techniques, this volume explores computational docking, quantitative structure-activity relationship (QSAR), peptide synthesis, labeling of peptides and proteins with fluorescent labels, DNA-microarray, zebrafish model for drug screening, and other analytical screening and biological assays that are routinely used during the drug discovery process. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and accessible, Drug Design and Discovery: Methods and Protocols serve as a vital laboratory reference for pharmaceutical chemists, medicinal chemists, and pharmacologists as well as for molecular biologists
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