Plant Immunity Methods and Protocols
A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes. In Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstandin...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2011, 2011
|
| Edition: | 1st ed. 2011 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression
- Fragment Complementation and Co-Immunoprecipitation Assays for Understanding R Protein Structure and Function
- Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
- Biochemical Purification of Native Immune Protein Complexes
- Chromatin Immunoprecipitation (ChIP) to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity
- Dose Response to and Systemic Movement of Dexamethasone in the GVG Inducible Transgene System in Arabidopsis
- Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis
- Constructing Haustorium-Specific cDNA Libraries from Rust Fungi
- Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes
- Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains
- A Straightforward Protocol for Electro-Transformation of Phytophthora capsici Zoospores
- Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis, a Model Oomycete Pathogen of Arabidopsis
- Assaying Effector Function In planta Using Double-Barreled Particle Bombardment
- Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast
- Purification of Effector-Target Protein Complexes via Transient Expression in N. benthamiana
- Imaging Fluorescently-Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue
- Immunolocalization of Pathogen Effectors
- Laser Capture Microdissection of Nematode Feeding Cells
- Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling
- Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites
- Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins