Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases Methods and Protocols

Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Me...

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Bibliographic Details
Other Authors: Nieden, Nicole I. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2011, 2011
Edition:1st ed. 2011
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Embryonic Stem Cells for Osteo-Degenerative Diseases
  • Methods for Culturing Mouse and Human Embryonic Stem Cells
  • Serum-Free and Feeder free Culture Conditions for Human Embryonic Stem Cells
  • Functional Assays for Human Embryonic Stem Cell Pluripotency
  • Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells
  • Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters
  • Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives
  • Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers
  • Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology
  • Methods for Embryoid Body Formation: The Microwell Approach
  • Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview
  • Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells
  • Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the “Raclure” Method
  • Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes
  • Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds
  • Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells
  • Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield
  • Identification of Osteoclasts in Culture
  • Analysis of Glycosaminoglycans in Stem Cell Glycomics
  • Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent
  • Measurement and Illustration of Immune Interaction after Stem Cell Transplantation