Light Microscopy Methods and Protocols

Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques,...

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Bibliographic Details
Other Authors: Chiarini-Garcia, Hélio (Editor), Melo, Rossana C. N. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2011, 2011
Edition:1st ed. 2011
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Glycol methacrylate embedding for improved morphological, morphometrical and immunohistochemical investigations under light microscopy: testes as a model
  • Histological processing of teeth and periodontal tissues for light microscopy analysis
  • Large plant samples: how to process for 2-hydroxyethyl methacrylate embedding?
  • Image cytometry: nuclear and chromosomal DNA quantification
  • Histological approaches to study tissue parasitism during Trypanosoma cruzi infection
  • Intravital microscopy to study leukocyte recruitment in vivo
  • Introduction to fluorescence microscopy
  • Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals
  • Imaging lipid bodies within leukocytes with different light microscopy techniques
  • EicosaCell – An immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites
  • Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer
  • Imaging calcium sparks in cardiac myocytes
  • Light microscopy in aquatic ecology: methods for plankton communities studies
  • Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedding samples