PCR Protocols
Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and trick...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2011, 2011
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| Edition: | 3rd ed. 2011 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Setup of a PCR Laboratory
- Long Range PCR with a DNA Polymerase Fusion
- Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures
- Lariat-Dependent Nested PCR for Flanking Sequence Determination
- CODEHOP PCR and CODEHOP PCR Primer Design
- Sequencing of Difficult DNA Regions by SAM Sequencing
- A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis
- Quantitation of MicroRNAs by Real-Time RT-qPCR
- High-Throughput SuperSAGE
- Deep Cap Analysis of Gene Expression (Deep CAGE)
- Linking Emulsion PCR Haplotype Analysis
- PAP-LMPCR: An Improved, Sequence-Selective Method for the In vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure
- The Many Faces of MLPA
- Assessing Gene-Specific Methylation Using HRM-Based Analysis
- Alu PCR
- Asynchronous PCR
- Novel Applications of PCR through the Use of DNA Substrates
- Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support
- Application of Blocking Oligonucleotides to Improve Signal to Noise Ratio in a PCR
- Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis
- Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries
- GLOBE: Analysis of DNA-Protein Interaction Analysis
- PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues
- Nucleotide Exchange and Excision Technology (NExT) DNA Shuffling and Directed Evolution