LC-MS/MS in Proteomics Methods and Applications
With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global sca...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2010, 2010
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| Edition: | 1st ed. 2010 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Overviews of Techniques and Approaches in LC-MS-Based Proteomics
- Approaches and Applications of Quantitative LC-MS for Proteomics and Activitomics
- Overview of Quantitative LC-MS Techniques for Proteomics and Activitomics
- Instrumentation for LC-MS/MS in Proteomics
- Bioinformatics for LC-MS/MS-Based Proteomics
- Analysis of Post-translational Modifications by LC-MS/MS
- Structural Analysis of Post-Translational Modifications
- In-Depth Analysis of Protein Phosphorylation by Multidimensional Ion Exchange Chromatography and Mass Spectrometry
- Mapping of Phosphorylation Sites by LC-MS/MS
- Analysis of Carbohydrates on Proteins by Offline Normal-Phase Liquid Chromatography MALDI-TOF/TOF-MS/MS
- Techniques for Quantitative LC-MS
- Selected Reaction Monitoring Applied to Quantitative Proteomics
- Basic Design of MRM Assays for Peptide Quantification
- Proteome-Wide Quantitation by SILAC
- Quantification of Proteins by iTRAQ
- Quantification of Proteins by Label-Free LC-MS/MS
- Analysis of Protein Complexes and Organelles
- Protocol for Quantitative Proteomics of Cellular Membranes and Membrane Rafts
- Organelle Proteomics by Label-Free and SILAC-Based Protein Correlation Profiling
- Mapping Protein–Protein Interactions by Quantitative Proteomics
- Analysis of Biological Fluids and Clinical Samples
- Analysis of Serum Proteins by LC-MS/MS
- Urinary Proteome Profiling Using 2D-DIGE and LC-MS/MS
- Analysis of Peptides in Biological Fluids by LC-MS/MS
- Novel Applications of LC-MS
- Quantification of Protein Kinase Activities by LC-MS
- A Protocol for Top-Down Proteomics Using HPLC and ETD/PTR-MS.