Plant Reverse Genetics Methods and Protocols

After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gen...

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Bibliographic Details
Other Authors: Pereira, Andy (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2011, 2011
Edition:1st ed. 2011
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Analysis of High-Throughput Sequencing Data
  • Identification of Plant MicroRNAs Using Expressed Sequence Tag Analysis
  • Microarray Data Analysis
  • Setting Up Reverse Transcription Quantitative-PCR Experiments
  • Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species
  • Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses
  • Full-Length cDNA Overexpressor Gene Hunting System (FOX Hunting System)
  • Activation Tagging with En/Spm-I/dSpm Transposons in Arabidopsis
  • Activation Tagging and Insertional Mutagenesis in Barley
  • Methods for Rice Phenomics Studies
  • Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice
  • Transposon Insertional Mutagenesis in Rice
  • Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants
  • Screening Arabidopsis Genotypes for Drought Stress Resistance
  • Protein Tagging for Chromatin Immunoprecipitation (ChIP) from Arabidopsis
  • Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions
  • Plant Metabolomics by GC-MS and Differential Analysis
  • Gramene Database: A Hub for Comparative Plant Genomics