Clostridium difficile Methods and Protocols

Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and P...

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Bibliographic Details
Other Authors: Mullany, Peter (Editor), Roberts, Adam P. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2010, 2010
Edition:1st ed. 2010
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • to Clostridium difficile and the Disease It Causes
  • Clostridium difficile: No Longer an Enigmatic Pathogen?
  • Clostridium difficile and the Disease It Causes
  • Isolation and Culture Techniques
  • Clostridium difficile Isolation and Culture Techniques
  • Methods for Typing and Epidemiological Studies
  • Molecular Typing Methods for Clostridium difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping
  • Clostridium difficile Toxinotyping
  • Multilocus Sequence Typing for Clostridium difficile
  • Biochemistry of the Organism
  • Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes
  • Dissecting the Cell Surface
  • Human Intestinal Epithelial Response(s) to Clostridium difficile
  • Genomics
  • Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays
  • Development of Systems for Genetic Analysis of the Organism
  • ClosTron-Targeted Mutagenesis
  • Methods for Gene Cloning and Targeted Mutagenesis
  • Transposon Mutagenesis in Clostridium difficile
  • Animal Models of Disease
  • Refinement of the Hamster Model of Clostridium difficile Disease
  • Methods for Working with the Mouse Model