Tissue Proteomics Pathways, Biomarkers, and Drug Discovery
With the completion of human genome sequencing, the field of proteomics has grown vital to biomedical research. Among the array of protocols developed to study the human proteome, many protocols examine proteins derived specifically from cell lines, body fluids or human tissues - not necessarily com...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2008, 2008
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| Edition: | 1st ed. 2008 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Preface
- Contributors
- 1 Capabilities Using 2-D DIGE in Proteomics Research: The New Gold Standard for 2-D Gel ElectrophoresisChristine R. Rozanas and Stacey M. Loyland
- 2 Protein Carbohydrate Analysis: Gel-Based Staining, Liquid Chromatography, Mass Spectrometry, and Microarray ScreeningInjae Shin, Alina D. Zamfir, and Bin Ye
- 3 Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry for Protein and Peptide Profiling of Body FluidsAudrey Gagnon, Qian Shi, and Bin Ye
- 4 A Novel Approach Using MALDI-TOF/TOF Mass Spectrometry and Prestructured Sample Supports (AnchorChip Technology) for Proteomic Profiling and Protein IdentificationSau-Mei Leung and Rebecca L. Pitts
- 5 Automated Laser Capture Microdissection for Tissue ProteomicsAdrianna S. Rodriguez, Benjamin H. Espina, Virginia Espina, and Lance A. Liotta
- 6 Tissue Microarrays: An OverviewRajiv Dhir
- 7 Frozen Protein ArraysStephen M. Hewitt and Robert A. Star
- 8 Reverse Phase Protein Microarrays for Theranostics and Patient-Tailored TherapyVirginia Espina, Julia Wulfkuhle, Valerie S. Calvert, Lance A. Liotta, and Emanuel F. Petricoin III
- 9 Antibody Arrays for Determination of Relative Protein AbundancesGrigoriy S. Chaga
- 10 SH2 Domain-Based Tyrosine Phosphorylation ArrayXin Jiang, Lesile Roth, Stephanie Han, and Xianqiang Li
- 11 Immunoregulomics: A Serum Autoantibody-Based Platform for Transcription Factor ProfilingOliver W. Tassinari, Margarita Aponte, Robert J. Caiazzo Jr., and Brian C.-S. Liu
- 12 The “Reverse Capture” Autoantibody Microarray: An Innovative Approach to Profiling the Autoantibody Response to Tissue-Derived Native AntigensJoshua R. Ehrlich, Liangdan Tang, Robert J. Caiazzo Jr., Daniel W. Cramer, Shu-Kay Ng, Shu-Wing Ng, and Brian C.-S. Liu
- 13 Biorepository Standards andProtocols for Collecting, Processing, and Storing Human TissuesDean Troyer