SUMO Protocols
While the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. In SUMO Protocols, leading experts present a collection of methods intended to h...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2009, 2009
|
| Edition: | 1st ed. 2009 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- The SUMO System: An Overview
- Identification of Sumo Targets
- Identification of SUMO Target Proteins by Quantitative Proteomics
- Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry
- Identification of SUMO Targets Through In Vitro Expression Cloning
- Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS)
- In Vivo Detection and Characterization of Sumoylation Targets in Saccharomyces cerevisiae
- Identification Of Sumo Interactors
- Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis
- Identification of SUMO-Binding Motifs by NMR
- Functional Analysis Of Sumoylation In Vivo
- Regulation of Transcription Factor Activity by SUMO Modification
- Characterization of the Effects and Functions of Sumoylation Through Rapamycin-Mediated Heterodimerization
- Biochemical Studies Of Sumo Conjugation
- Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation
- Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
- Preparation of Sumoylated Substrates for Biochemical Analysis
- Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli
- Biochemical Studies of Sumo Deconjugation
- Preparation of SUMO Proteases and Kinetic Analysis Using Endogenous Substrates
- An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage
- FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities
- Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay
- Manipulation And Alternative Uses Of The Sumo System
- Inhibition of the SUMO Pathway by Gam1
- SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems