Potassium Channels Methods and Protocols
Due to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels. In Potassium Channels: Methods and Protocols,...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2009, 2009
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| Edition: | 1st ed. 2009 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Determination of Phosphoinositide Binding to K+ Channel Subunits Using a Protein—Lipid Overlay Assay
- Protein Complex Analysis of Native Brain Potassium Channels by Proteomics
- Electrophysiological Techniques For The Study Of Potassium Channel Function
- Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp Technique
- Whole-Cell Recording Using the Perforated Patch Clamp Technique
- Recording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration
- Planar Patch Clamp: Advances in Electrophysiology
- Analysing Steroid Modulation of BKCa Channels Reconstituted into Planar Lipid Bilayers
- Optical Techniques For The Study Of Potassium Channel Function And Interactions
- Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly
- The Use of FRET Microscopy to ElucidateSteady State Channel Conformational Rearrangements and G Protein Interaction with the GIRK Channels
- Methods For Studying Potassium Channel Transcription And Mrna Splicing
- Identifying Transcriptional Regulatory Regions Using Reporter Genes and DNA—Protein Interactions by Chromatin Immunoprecipitation
- Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries
- Cloning of Potassium Channel Splice Variants from Tissues and Cells
- Methods for Studying the Cell Biology of Potassium Channel Proteins
- Chemiluminescence Assays to Investigate Membrane Expression and Clathrin-Mediated Endocytosis of KATP Channels
- Investigation of KATP Channel Endocytosis by Immunofluorescence
- Investigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting
- Lipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisation of Cholesterol-Enriched Lipid Rafts
- The Voltage-Clamp Fluorometry Technique
- Methods For Studying Potassium Channelopathies And Pharmacological Modulators
- Identification of Mutations in the Kir6.2 Subunit of the KATP Channel
- Modulation of Potassium Ion Channel Proteins Utilising Antibodies
- Fluorescence-Based Tl+?Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca2+?Activated K+ Channel Modulators
- Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Opening Properties
- Recording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch-Clamp Technique