Baculovirus and Insect Cell Expression Protocols

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind o...

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Bibliographic Details
Other Authors: Murhammer, David W. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2007, 2007
Edition:2nd ed. 2007
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Methods for Gene Silencing With RNAi
  • Using the Baculovirus/Insect Cell System to Study Apoptosis
  • Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells
  • Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae
  • Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insect Cell System
  • Recombinant Protein Production with Transformed Insect Cells
  • Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
  • Transformation of Drosophila Cell Lines
  • Transforming Lepidopteran Insect Cells for Improved Protein Processing
  • Baculovirus Development and Production for Use as Insecticides
  • to the Use of Baculoviruses as Biological Insecticides
  • Baculovirus Insecticide Production in Insect Larvae
  • Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses
  • Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System
  • Monitoring and Visualization of Baculovirus Infection UsingGreen Fluorescent Protein Strategy
  • Alternative Bioreactor Strategy for Probing Infection and Production
  • Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior
  • Baculovirus Molecular Biology/Development of Recombinant Baculoviruses
  • to Baculovirus Molecular Biology
  • Baculovirus Transfer Vectors
  • Recombinant Baculovirus Isolation
  • Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System
  • Insect Cell Culture
  • Available Lepidopteran Insect Cell Lines
  • Lepidopteran Insect Cell Line Isolation from Insect Tissue
  • Development of Serum-Free Media for Lepidopteran Insect Cell Lines
  • Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses
  • Protein Production with Recombinant Baculoviruses
  • Small-Scale Protein Production With the Baculovirus Expression Vector System
  • Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
  • Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors