Baculovirus and Insect Cell Expression Protocols
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind o...
Other Authors: | |
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Format: | eBook |
Language: | English |
Published: |
Totowa, NJ
Humana
2007, 2007
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Edition: | 2nd ed. 2007 |
Series: | Methods in Molecular Biology
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Subjects: | |
Online Access: | |
Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Methods for Gene Silencing With RNAi
- Using the Baculovirus/Insect Cell System to Study Apoptosis
- Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells
- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae
- Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insect Cell System
- Recombinant Protein Production with Transformed Insect Cells
- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
- Transformation of Drosophila Cell Lines
- Transforming Lepidopteran Insect Cells for Improved Protein Processing
- Baculovirus Development and Production for Use as Insecticides
- to the Use of Baculoviruses as Biological Insecticides
- Baculovirus Insecticide Production in Insect Larvae
- Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses
- Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System
- Monitoring and Visualization of Baculovirus Infection UsingGreen Fluorescent Protein Strategy
- Alternative Bioreactor Strategy for Probing Infection and Production
- Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior
- Baculovirus Molecular Biology/Development of Recombinant Baculoviruses
- to Baculovirus Molecular Biology
- Baculovirus Transfer Vectors
- Recombinant Baculovirus Isolation
- Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System
- Insect Cell Culture
- Available Lepidopteran Insect Cell Lines
- Lepidopteran Insect Cell Line Isolation from Insect Tissue
- Development of Serum-Free Media for Lepidopteran Insect Cell Lines
- Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses
- Protein Production with Recombinant Baculoviruses
- Small-Scale Protein Production With the Baculovirus Expression Vector System
- Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
- Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors