Immunocytochemical Methods and Protocols
The landscape of immunocytochemistry has significantly evolved in past years. Once a laborious technique with limited application, the introduction of new detection systems and instrumentation has facilitated the widespread use of immunocytochemical methods. In the post-genomic era, the use of immun...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2010, 2010
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| Edition: | 3rd ed. 2010 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Antibody Preparation
- Overview of Antibodies for Immunochemistry
- to the Purification of Antibodies
- Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration
- Antibody Purification: Ion-Exchange Chromatography
- Antibody Purification: Affinity Chromatography – Protein A and Protein G Sepharose
- Conjugation of Fluorochromes to Antibodies
- Biotinylation of Antibodies
- Tissue Preparation for Light Microscopic Analysis
- Cell Fixatives for Immunostaining
- Permeabilization of Cell Membranes
- Preparation of Frozen Sections for Analysis
- Processing of Cytological Specimens
- Processing of Tissue Culture Cells
- Processing of Tissue Specimens
- Heat-Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections
- Bright Field Detection Systems
- Fluorochromes: Properties and Characteristics
- Direct Immunofluorescent Labeling of Cells
- Use of Immunogold with Silver Enhancement
- Fluorescence-Activated Cell Sorter (FACS) Analyses
- Overview of Flow Cytometry and Fluorescent Probes for Flow Cytometry
- Tissue Disaggregation
- Indirect Immunofluorescent Labeling of Viable Cells
- Indirect Immunofluorescent Labeling of Fixed Cells
- Fluorescent Labeling of DNA
- Deparaffinization and Processing of Pathologic Material
- Colloidal Gold Detection Systems for Electron Microscopic Analysis
- Fixation and Embedding
- Preparation of Colloidal Gold
- Conjugation of Colloidal Gold to Proteins
- Colloidal Gold/Streptavidin Methods
- Pre-embedding Labeling Methods
- Postembedding Labeling Methods
- The Clinical Laboratory
- The Clinical Immunohistochemistry Laboratory: Regulations and Troubleshooting Guidelines
- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells
- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells
- Fluorescent Visualization of Macromolecules in Drosophila Whole Mounts
- Overview of Conventional Fluorescence Photomicrography
- Overview of Confocal Microscopy
- Overview of Laser Microbeam Applications as Related to Antibody Targeting
- Immuno-Laser Capture Microdissection of Rat Brain Neurons for Real Time Quantitative PCR
- Overview of Antigen Detection Through Enzymatic Activity
- The Peroxidase–Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
- The Avidin–Biotin Complex (ABC) Method and Other Avidin–Biotin Binding Methods
- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue
- Multiple Antigen Immunostaining Procedures
- ImmunoenzymaticQuantitative Analysis of Antigens Expressed on the Cell Surface (Cell-ELISA)