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02031nmm a2200253 u 4500 |
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120214 ||| eng |
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|a 9789264122642
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|a Test No. 456: H295R Steroidogenesis Assay
|h Elektronische Ressource
|c Organisation for Economic Co-operation and Development
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|a Essai n° 456 : Essai de stéroïdogenèse H295R
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260 |
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|a Paris
|b OECD Publishing
|c 2023
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300 |
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|a 27 p
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653 |
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|a Environment
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710 |
2 |
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|a Organisation for Economic Co-operation and Development
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|a eng
|2 ISO 639-2
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989 |
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|b OECD
|a OECD Books and Papers
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490 |
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|a OECD Guidelines for the Testing of Chemicals, Section 4
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|a 10.1787/9789264122642-en
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856 |
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|a oecd-ilibrary.org
|u https://doi.org/10.1787/9789264122642-en
|x Verlag
|3 Volltext
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082 |
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|a 363
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520 |
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|a This Test Guideline describes an in vitro screen for chemical effects on steroidogenesis, specifically the production of 17ß-estradiol (E2) and testosterone (T). The human H295R adreno-carcinoma cell line, used for the assay, expresses genes that encode for all the key enzymes for steroidogenesis. After an acclimation period of 24 h in multi-well plates, cells are exposed for 48 h to seven concentrations of the test chemical in at least triplicate. Solvent and a known inhibitor and inducer of hormone production are run at a fixed concentration as negative and positive controls. At the end of the exposure period, cell viability in each well is analyzed. Concentrations of hormones in the medium can be measured using a variety of methods including commercially available hormone measurement kits and/or instrumental techniques such as liquid chromatography-mass spectrometry. Data are expressed as fold change relative to the solvent control and the Lowest-Observed-Effect-Concentration. If the assay is negative, the highest concentration tested is reported as the No-Observed-Effect-Concentration
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