Transgenic Plants Methods and Protocols
Transgenic plants serve as essential tools for research in plant biology and are the basis of modern agricultural biotechnology. In Transgenic Plants: Methods and Protocols, leading scientists describe readily reproducible techniques they have perfected for the genetic transformation and regeneratio...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2004, 2004
|
| Edition: | 1st ed. 2004 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Transgenic Plants: An Historical Perspective
- Transformation
- Plant Transformation: Agrobacterium-Mediated Gene Transfer
- Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation
- Stable Transformation of Plant Cells by Particle Bombardment/Biolistics
- Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts
- Floral Dip: Agrobacterium-Mediated Germ Line Transformation
- Production of Transgenic Crops by the Floral-Dip Method
- Chloroplast Genetic Engineering to Improve Agronomic Traits
- Regeneration
- Organogenesis From Transformed Tomato Explants
- Genetic Transformation of Conifers Utilizing Somatic Embryogenesis
- Regeneration of Transgenic Cassava From Transformed Embryogenic Tissues
- Genetic Transformation of Mature Citrus Plants
- Selection
- Selectable Markers: Antibiotic and Herbicide Resistance
- Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection
- Green Fluorescent Protein Quantification in Whole Plants
- Positive Selection
- Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems
- Simple and Efficient Removal of Marker Genes From Plastids by Homologous Recombination
- Transgene Integration, Expression, and Localization
- The Study of Transgene Copy Number and Organization
- Analysis of Gene Expression in Transgenic Plants
- Transgene Integration: Use of Matrix Attachment Regions
- Fluorescence In Situ Hybridization to Localize Transgenes in Plant Chromosomes
- Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction
- Risk Assessment
- Agrobacterium Persistence in Plant Tissues After Transformation
- Transgene Dispersal Through Pollen
- Transgenic Crops
- Transgenic Crops: The Current and Next Generations
- Comparative Development and Impact of Transgenic Papayas in Hawaii, Jamaica, and Venezuela