Germ Cell Protocols Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning
Germ cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a s...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2004, 2004
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| Edition: | 1st ed. 2004 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos
- Analysis of Germline Chromatin Silencing by Double-Stranded RNA-Mediated Interference (RNAi) in Caenorhabditis elegans
- cDNA Subtraction and Cloning in the Field of Trophoblast/Placental Development
- PCR-Based Cloning and Differential Screening of RNAs from Xenopus Primordial Germ Cells
- Differentiating Embryonic Stem Cells into Embryoid Bodies
- The Derivation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation
- Using Multiphoton Excitation to Explore the Murky Depths of Developing Embryos
- Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes
- A Method for Producing Cloned Pigs by Using Somatic Cells as Donors
- Pronuclear Microinjection of Mouse Zygotes
- Parthenogenesis and Nuclear Transfer in Rabbit Oocytes
- Nuclear Transfer in Cattle
- In Vitro Fertilization and Embryo Transfer in Felids
- Production of Chimeric Chickens
- Production of Haploid and Diploid Androgenetic Zebrafish
- Construction and Detection of Fluorescent, Germline Transgenic Zebrafish
- Production of Zebrafish Germline Chimeras from Cultured Cells
- Studying Nuclear Reprogramming with Cell Hybrids
- Cytoplasmic Transfer in Mammalian Eggs and Embryos
- Conventional Technologies for Cryopreservation of Human Oocytes and Embryos
- Vitrifying and Warming of Human Oocytes, Embryos, and Blastocysts
- Microfluidic Removal of Cumulus Cells from Mammalian Zygotes
- Mammalian Embryo Culture in a Microfluidic Device
- Application of Alginate Gels to the Study of Mammalian Development
- Using TUNEL in Combination with an Active Caspase-3 Immunoassay to Identify Cells Undergoing Apoptosis in Preimplantation Mammalian Embryos