mRNA Processing and Metabolism Methods and Protocols
mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism. Drawing on recent advances...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2004, 2004
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| Edition: | 1st ed. 2004 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevisiae
- Identifying PhosphoCTD-Associating Proteins
- Imaging Alternative Splicing in Living Cells
- Tobramycin Affinity Tag Purification of Spliceosomes
- Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing
- Methods for the Analysis of Adenosine-to-Inosine Editing in RNA
- Assaying Nuclear Messenger RNA Export in Human Cells
- RNA Unwinding Assay for DExD/H-Box RNA Helicases
- Approaches for Monitoring Nuclear Translation
- Immunopurification and Analysis of Protein and RNA Components of mRNP in Mammalian Cells
- Gene Expression Analysis of Messenger RNP Complexes
- Using the ?N Peptide to Tether Proteins to RNAs
- An Efficient System for Cap- and Poly(A)-Dependent Translation In Vitro
- A Poly(A) Tail-Responsive In Vitro System for Cap- or IRES-Driven Translation From HeLa Cells
- Assessing Messenger RNA Decapping in Cellular Extracts
- Analysis of RNA Exonucleolytic Activities in Cellular Extracts
- Application of Ligation-Mediated Reverse Transcription Polymerase Chain Reaction to the Identification of In Vivo Endonuclease-Generated Messenger RNA Decay Intermediates
- Biochemical Dissection of RNA Silencing in Plants
- RNA Interference of mRNA Processing Factors in Drosophila S2 Cells
- RNA Interference by Short Hairpin RNAs Expressed in Vertebrate Cells