Molecular Diagnosis of Cancer Methods and Protocols

The growing tide of novel therapeutics that target molecules within a specific individual's cancer cells-over 300 such agents are currently in phase I or II clinical trials-makes it essential to employ appropriate diagnostic methods and approaches when selecting patients for treatment. In Molec...

Full description

Bibliographic Details
Other Authors: Roulston, Joseph E. (Editor), Bartlett, John M. S. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2004, 2004
Edition:2nd ed. 2004
Series:Methods in Molecular Medicine
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Prognostic and Predictive Factors
  • Assessment of Predictive Values of Tumor Markers
  • Quality Assurance of Predictive Markers in Breast Cancer
  • Extraction of Nucleic Acid Templates
  • Microdissection and Extraction of DNA From Archival Tissue
  • Fluorescence In Situ Hybridization
  • HER2 FISH in Breast Cancer
  • Fluorescence In Situ Hybridization for BCR-ABL
  • UroVysion™ Multiprobe FISH in Urinary Cytology
  • Chromogenic In Situ Hybridization in Tumor Pathology
  • Comparative Genomic Hybridization and Fluorescence In Situ Hybridization in Chronic Lymphocytic Leukemia
  • Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridization
  • A Nested RT-PCR Assay to Detect BCR/abl
  • TP53 Mutation Detection by SSCP and Sequencing
  • PCR Diagnosis of T-Cell Lymphoma in Paraffin-Embedded Bone Marrow Biopsies
  • Circulating DNA Analysis
  • Microsatellite Instability
  • Diagnostic and Prognostic Significance of the Methylation Status of Myf-3 in Lymphoproliferative Disorders
  • Quantitative Analysis of PRAME for Detection of Minimal Residual Disease in Leukemia
  • Determination of Cyclin D1 Expression by Quantitative Real-Time, Reverse-Transcriptase Polymerase Chain Reaction
  • Detection of Telomerase hTERT Gene Expression and Its Splice Variants by RT-PCR
  • Detection of Telomerase Enzyme Activity by TRAP Assay
  • Identification of TP53 Mutations in Human Cancers Using Oligonucleotide Microarrays
  • Detection of K-ras Mutations by a Microelectronic DNA Chip
  • Microarray-Based CGH in Cancer
  • Tissue Microarrays