Membrane Protein Protocols Expression, Purification, and Characterization
Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at major...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2003, 2003
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| Edition: | 1st ed. 2003 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Purification of Porins from Mycobacterium smegmatis
- Isolation of the Melanocortin 5 Receptor
- Purification of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis, by Affinity-Peptide Chromatography
- Purification of Phosphatidylglycerophosphate Synthase from Cultured Mammalian Cells
- Purification of Pancreastatin Receptor from Rat Liver Membranes
- Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes
- Isolation of Lipid Raft-Associated Proteolipids
- Purification of Membrane-Bound Catechol-O-Methyltransferase
- Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrane
- Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase
- Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes
- Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I
- Structural Analysis of Membrane Proteins
- Crystallization in Lipidic Cubic Phases
- Optical Biosensor Assay Using Retroviral Receptor Pseudotypes
- Expression of Membrane Proteins
- Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b5 in Escherichia coli
- Dihydroorotate Dehydrogenase of Escherichia coli
- A General Approach for Heterologous Membrane Protein Expression in Escherichia coli
- Expression of Membrane-Bound Iron-Sulfur Proteins
- Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris
- Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells
- Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells
- Detergent Selection in Membrane Protein Purification
- Preparation of Glycerol Facilitator for Protein Structure and Folding Studies in Solution
- Solubilization of Chemokine Receptors from Cell Membranes
- A Systematic Approach for the Solubilization of the Integral Membrane Protein Lysophospholipid
- Membrane Protein Purification
- Purification of Omp50, a Minor Porin of Campylobacter jejuni