p53 Protocols
Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal its unique structural features and biochemical functions. In p53 Protocols, Sumitra and Swati Palit Deb have assembled an indispensable collection of novel techniques that have proven most useful for studying t...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2003, 2003
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| Edition: | 1st ed. 2003 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Adenovirus Expressing p53
- Purification of Recombinant p53 from Sf9 Insect Cells
- Generation and Characterization of p53 Mutant Mice
- Identification of p53 Target Genes by Fluorescent Differential Display
- p53-Induced Gene Expression Analysis
- Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Using SYBR® Green I
- Transactivation and Transrepression Studies with p53
- Methods to Study p53-Repressed Promoters
- Interaction of p53 with Cellular Proteins
- Interaction of p53 with the Adenovirus E1B-55 kDa Protein
- In Vivo Footprinting and DNA Affinity Chromatography for Analysis of p53 DNA Binding Ability
- Signaling to p53
- Detection of Mitochondrial Localization of p53
- Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress
- Mutational Analysis of p53 in Human Tumors
- Mutational Analysis of p53 in Human Tumors
- Flow Cytometric Analysis of p53-Induced Apoptosis
- Flow Cytometric Analysis of MDM2-Mediated Growth Arrest