Platelets and Megakaryocytes Volume 1: Functional Assays
Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2004, 2004
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| Edition: | 1st ed. 2004 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Platelet Functional Assays
- Effects of Anticoagulants Used During Blood Collection on Human Platelet Function
- Preparation of Washed Platelet Suspensions From Human and Rodent Blood
- Platelet Counting
- Electron Microscopy Methods for Studying Platelet Structure and Function
- Platelet Aggregation
- Platelet Aggregation in Whole Blood
- Secretion From Dense Granules
- Platelet Dense-Granule Secretion
- Analysis of the Releasable Nucleotides of Platelets
- Studies of Secretion Using Permeabilized Platelets
- Measurement of Platelet Arachidonic Acid Metabolism
- Measurement of the Platelet Procoagulant Response
- Platelet Adhesion Assays Performed Under Static Conditions
- Platelet Adhesion Assays Under Flow Using Matrix Protein-Coupled Adhesion Columns
- Techniques to Examine Platelet Adhesive Interactions Under Flow
- In Vivo Models of Platelet Function and Thrombosis
- Adhesion Between Platelets and Leukocytes or Endothelial Cells
- In Vitro Measurement of High-Shear Platelet Adhesion and Aggregation by the PFA-100®
- Flow-Cytometric Analysis of Platelet-Membrane Glycoprotein Expression and Platelet Activation
- Flow-Cytometric Analysis of Mouse Platelet Function
- Platelet Microparticles
- Megakaryocyte Functional Assays
- Isolation of Primary Megakaryocytes and Studies of Proplatelet Formation
- Isolation and Culture of Megakaryocyte Precursors
- Assays of Megakaryocyte Development
- Assays of Megakaryocyte Development
- Thrombopoietin Bioassay
- Culture of Megakaryocytic Cell Lines