Cancer Cell Signaling Methods and Protocols
The rapidly evolving nature of cell signaling research in cancer has necessitated a continuous updating of the methods used in its study, most recently to take into account the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together all the maj...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2003, 2003
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| Edition: | 1st ed. 2003 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions
- Genomic Rearrangements
- Mutational Analysis of the Androgen Receptor Using Laser Capture Microdissection and Direct Sequencing
- Clonality Analysis by T-Cell Receptor ? PCR and High-Resolution Electrophoresis in the Diagnosis of Cutaneous T-Cell Lymphoma (CTCL)
- Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay
- Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells
- Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD)
- Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model
- Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer
- Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes
- Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes
- Protein Interactions
- Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology
- Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay
- Manipulation and Detection of Survival Signals
- Functional Analysis of the Antimitogenic Activity of Tumor Suppressors
- Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination
- Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells
- The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress
- Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression
- Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells
- TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s)
- Manipulation and Edtection of Oncogenic Signals
- Kinetworks™ Protein Kinase Multiblot Analysis
- Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers
- Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay