Blood'Brain Barrier Biology and Research Protocols

Despite the fact that the breakdown of the blood-brain barrier occurs in many brain diseases and is an important factor in mortality, the molecular mechanisms involved in the breakdown are not completely understood. In The Blood-Brain Barrier: Biology and Research Protocols, leading researchers and...

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Bibliographic Details
Other Authors: Nag, Sukriti (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2003, 2003
Edition:1st ed. 2003
Series:Methods in Molecular Medicine
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • A Review of Blood-Brain Barrier Transport Techniques
  • In Situ Brain Perfusion Technique
  • Brain Efflux Index Method
  • Transport Studies Using Membrane Vesicles
  • Drug Transport Studies Using Quantitative Microdialysis
  • Single-Pass Dual-Label Indicator Method
  • Protein Transport in Cerebral Endothelium
  • Transport Across the Choroid Plexuses In Vivo and In Vitro
  • In Vitro Techniques
  • An Overview of In Vitro Techniques for Blood-Brain Barrier Studies
  • Isolation and Characterization of Human Brain Endothelial Cells
  • Human Brain Microvessel Endothelial Cell and Leukocyte Interactions
  • Development and Characterization of Immortalized Cerebral Endothelial Cell Lines
  • Isolation and Characterization of Retinal Endothelial Cells
  • Isolation and Characterization of Cerebral Microvascular Pericytes
  • Molecular Techniques
  • Molecular Biology of the Blood-Brain Barrier
  • Blood-Brain Barrier Genomics
  • Dynamic In Vitro Model of the Blood-Brain Barrier
  • Tissue Techniques
  • Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels
  • Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry
  • Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions
  • Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy
  • Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy
  • Blood-Brain Barrier Permeability Techniques
  • Pathophysiology of Blood-Brain Barrier Breakdown
  • Pial Vessel Permeability to Tracers Using Cranial Windows
  • Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry
  • Ultracytochemical Studies of the Compromised Blood-Brain Barrier
  • Immunogold Detection of Microvascular Proteins in the Compromised Blood-Brain Barrier
  • Measurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [14C]-Labeled Tracers
  • Blood-Brain Barrier Transport Techniques
  • Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells
  • mRNA Detection in Cerebral Vessels by Nonradioactive In Situ Hybridization
  • Proteomics of Brain Endothelium
  • Immunoblot Detection of Brain Vascular Proteins
  • Immunohistochemical Detection of Endothelial Proteins
  • Genetically Altered Mice
  • Cerebral Vascular Function in Genetically Altered Mice
  • Generation of Transgenic Mice by Pronuclear Injection
  • Mouse Genome Modification