Opioid Research Methods and Protocols
Opioid research has grown enormously over the last decade, and with it, the need to apply multiple techniques from a variety of disciplines. In Opioid Research: Methods and Protocols, expert researchers and physicians provide a comprehensive collection of the major cross-disciplinary methods and pro...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2003, 2003
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| Edition: | 1st ed. 2003 |
| Series: | Methods in Molecular Medicine
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Molecular Characterization of Opioid Receptors and Signaling Pathways
- Molecular Cloning of Opioid Receptors by cDNA Library Screening
- Expression of Opioid Receptors in Mammalian Cell Lines
- Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cells
- Analysis of Opioid-Induced Kinase Activation
- Study of Opioid Receptor Phosphorylation Using Cell-Labeling Method with 32P-Orthrophosphate
- Opioid Receptor Coupling to GIRK Channels
- Identification of Alternatively Spliced Variants from Opioid Receptor Genes
- Mapping and Detection of Endogenous Opioids
- Immunohistochemical Localization of ?-, ?- and K-Opioid Receptors Within the Antinociceptive Brainstem Circuits
- In Situ Hybridization in Neural Tissues
- Quantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping Genes
- Gene Arrays and Proteomics
- Model Systems for Studies on Opioid Functions
- Opioid Receptor Oligomerization
- Recombinant Opioid Receptors
- Receptor Knock-Out and Gene Targeting
- Pharmacological Interventions at the Spinal Cord
- Opioid Tolerance in Adult and Neonatal Rats
- Animal Models of Neuropathic Pain
- Place Conditioning to Study Drug Reward and Aversion
- Opiate Self-Administration
- Clinical Applications of Opioids
- Acute, Chronic, and Cancer Pain
- Clinical Treatment of Opioid Addiction and Dependence