Bone Research Protocols
The past decade has witnessed the discovery of many genes with profound effects on bone. In Bone Research Protocols, recognized authorities with extensive hands-on experience present a wide-ranging catalog of laboratory techniques that will help researchers to investigate the mechanisms by which the...
| Other Authors: | , |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2003, 2003
|
| Edition: | 1st ed. 2003 |
| Series: | Methods in Molecular Medicine
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Culture of Osteolblasts and Osteocytes
- Human Osteoblast Culture
- Osteoblast Isolation from Murine Calvariae and Long Bones
- Mineralizing Fibroblast-Colony-Forming Assays
- Osteocyte Isolation and Culture
- Culture of Osteoclasts
- Isolated Osteoclast Cultures
- Primary Isolation and Culture of Chicken Osteoclasts
- Isolation and Purification of Rabbit Osteoclasts
- Generating Human Osteoclasts from Peripheral Blood
- Generating Human Osteoclasts In Vitro from Bone Marrow and Peripheral Blood
- Generating Murine Osteoclasts from Bone Marrow
- Osteoclast Formation in the Mouse Coculture Assay
- RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells
- Bone Organ Culture
- Analysis of Osteoclast Function in Mouse Calvarial Cultures
- Assessing Bone Formation Using Mouse Calvarial Organ Cultures
- Histological and Ultrastructural Techniques
- In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections
- Analysis of Gene Expression in Bone by Quantitative RT-PCR.
- Techniques for the Study of Apoptosis in Bone
- Protein Localization in Wax-Embedded and Frozen Sections of Bone Using Immunohistochemistry
- Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections
- Fluorescence Imaging of Bone-Resorbing Osteoclasts by Confocal Microscopy
- Bone Histomorphometry
- Transmission Electron Microscopy of Bone
- Scanning Electron Microscopy of Bone
- Imaging Techniques
- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents
- In Vivo Techniques
- Studies of Local Bone Remodeling
- Inflammation-Induced Osteoporosis
- Ovariectomy and Estrogen Replacement in Rodents
- Mechanical Loading Techniques
- Mechanical Testing of Bone Ex Vivo
- Bone Cell Responses to Fluid Flow
- Methods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture Models
- Molecular Analysis
- Extraction of Nucleic Acids from Bone