E. coli Shiga Toxin Methods and Protocols

Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experi...

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Bibliographic Details
Other Authors: Philpott, Dana (Editor), Ebel, Frank (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2003, 2003
Edition:1st ed. 2003
Series:Methods in Molecular Medicine
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections
  • Methods for Detection of STEC in Humans
  • Serological Methods for the Detection of STEC Infections
  • Detection and Characterization of STEC in Stool Samples Using PCR
  • Molecular Typing Methods for STEC
  • STEC in the Food Chain
  • STEC as a Veterinary Problem
  • Cellular Microbiology of STEC Infections
  • Analysis of Pathogenicity Islands of STEC
  • Generation of Isogenic Deletion Mutants of STEC
  • Generation of Monoclonal Antibodies Against Secreted Proteins of STEC
  • Microscopic Methods to Study STEC
  • Detection and Characterization of EHEC-Hemolysin
  • Shiga Toxin Receptor Glycolipid Binding
  • Methods for the Purification of Shiga Toxin 1
  • Methods for the Identification of Host Receptors for Shiga Toxin
  • Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport
  • Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin
  • Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways
  • Interaction of Shiga Toxin with Endothelial Cells
  • Shiga Toxin Interactions with the Intestinal Epithelium
  • Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes
  • Animal Models for STEC-Mediated Disease
  • Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC
  • Bovine Escherichia coli O157:H7 Infection Model