In Vitro Mutagenesis Protocols
In the post-genome era, in vitro mutagenesis has emerged as the critically important tool used by molecular biologists in establishing the functions of components of the proteome. In this second edition of In Vitro Mutagenesis Protocols, active researchers with proven track records describe in stepw...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2002, 2002
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| Edition: | 2nd ed. 2002 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Mutagenic Polymerase Chain Reaction of Protein-Coding Genes for In Vitro Evolution
- Phenotypic Expression of Polymerase Chain Reaction-Generated Random Mutations in a Foreign Gene After its Introduction into an Acinetobacter Chromosome by Natural Transformation
- Polymerase Chain Reaction-Mediated Mutagenesis in Sequences Resistant to Homogeneous Amplification
- Polymerase Chain Reaction-Based Signature-Tagged Mutagenesis
- High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction
- In Vitro Scanning-Saturation Mutagenesis
- Random Transposon Mutagenesis of Large DNA Molecules in Escherichia coli
- Random Chromosomal Gene Disruption Using Cassette Mutagenesis
- Transplacement Mutagenesis
- Preparation of Transposon Insertion Lines and Determination of Insertion Sites in Arabidopsis Genome
- Evolutionary Molecular Engineering by Random Elongation Mutagenesis
- Random Mutagenesis for Protein Breeding
- DNA Shuffling and Family Shuffling for In Vitro Gene Evolution
- Rapid and Reliable Site-Directed Mutagenesis Using Kunkel’s Approach
- Site-Directed Mutagenesis Using Altered /gb-Lactamase Specificity
- Site-Directed Mutagenesis Facilitated by DpnI Selection on Hemimethylated DNA
- Multiple Site-Directed Mutagenesis In Vitro
- Two-Stage Polymerase Chain Reaction Protocol Allowing Introduction of Multiple Mutations, Deletions, and Insertions, Using QuikChangeTM Site-Directed Mutagenesis
- Efficient and Accurate Site-Directed Mutagenesis of Large Plasmids
- Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis
- Site-Directed Mutagenesis Mediated by a Single Polymerase Chain Reaction Product
- Megaprimer Method for Polymerase Chain Reaction-Mediated Generation of Specific Mutations in DNA
- Generation of Epitope-Tagged Proteins by Inverse Polymerase Chain Reaction Mutagenesis
- Site-Directed Mutagenesis by Polymerase Chain Reaction
- Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction