Developmental Biology Protocols Volume I

In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. The second and third volumes in this seminal set complete today's widest-ranging...

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Bibliographic Details
Other Authors: Tuan, Rocky S. (Editor), Lo, Cecilia W. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2000, 2000
Edition:1st ed. 2000
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Developmental Biology Protocols
  • Systems: Production, Culture, and Storage
  • Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies
  • Large-Scale Culture and Preparation of Sea Urchin Embryos for Isolation of Transcriptional Regulatory Proteins
  • The Chick Embryo as a Model System for Analyzing Mechanisms of Development
  • Culture of Avian Embryos
  • Exo Ovo Culture of Avian Embryos
  • Culture of Preimplantation Mouse Embryos
  • In Vitro Culture of Rodent Embryos During the Early Postimplantation Period
  • Cryopreservation of Mouse Embryos
  • Developmental Pattern and Morphogenesis
  • Studying Head and Brain Development in Drosophila
  • Bioassays of Inductive Interactions in Amphibian Development
  • Gastrulation and Early Mesodermal Patterning in Vertebrates
  • Craniofacial Development of Avian and Rodent Embryos
  • Examination of the Axial Skeleton of Fetal Rodents
  • Cardiac Morphogenesis and Dysmorphogenesis
  • Embryo Structure and Function
  • Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos
  • Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos
  • Whole-Mount Immunolabeling of Embryos by Microinjection
  • Confocal Laser Scanning Microscopy Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos
  • Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscopy
  • Magnetic Resonance Imaging Analysis of Embryos
  • Optical Coherence Tomography Imaging in Developmental Biology
  • Ultrasound Backscatter Microscopy of Mouse Embryos
  • Use of Doppler Echocardiography to Monitor Embryonic Mouse Heart Function
  • Calcium Imaging in Cell-Cell Signaling
  • Acquisition, Display, and Analysis of Digital Three-Dimensional Time-Lapse (Four-Dimensional) Data Sets Using Free Software Applications
  • Cell Lineage Analysis
  • Cell Lineage Analysis
  • Cell Lineage Analysis
  • Laser Ablation and Fate Mapping
  • Photoablation of Cells Expressing ?-Galactosidase
  • Exo utero Surgery
  • Application of Viral Vectors in the Analysis of Development
  • Methods for Constructing and Producing Retroviral Vectors
  • Retroviral Gene Transduction in Limb Bud Micromass Cultures
  • Construction of Adenoviral Vectors
  • Application of Adenoviral Vectors
  • The Application of Adenoviral Vectors in the Study of Mammalian Cardiovascular Development
  • Retroviral Cell Lineage Analysis in the Developing Chick Heart
  • Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in Avian Embryos
  • Cell Lineage Analysis
  • Cell Lineage Analysis in Xenopus Embryos
  • Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryo
  • Carboxyfluorescein as a Marker at Both Light and Electron Microscope Levels to Follow Cell Lineage in the Embryo
  • Chimeras
  • Transplantation Chimeras
  • Interspecific Chimeras in Avian Embryos
  • Quail-Chick Transplantation in the Embryonic Limb Bud
  • Mouse Chimeras and the Analysis of Development
  • Cell Grafting and Fate Mapping of the Early-Somite-Stage Mouse Embryo
  • Interspecific Chimeras
  • Interspecific Mouse-Chick Chimeras
  • Mosaic Analysis in Caenorhabditis elegans
  • Experimental Manipulation of Embryos
  • Local Application of Bone Morphogenic Protein on Developing Chick Embryos Using a Fibrous Glass Matrix as a Carrier