Mycobacterium Tuberculosis Protocols
With one-third of the world's population infected with Mycobacterium tuberculosis, over two million people a year dying from tuberculosis, and the appearance of multidrug-resistant strains, the need to understand the biology of M. tuberculosis, and so to develop new interventions, has become ac...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2001, 2001
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| Edition: | 1st ed. 2001 |
| Series: | Methods in Molecular Medicine
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Plasmid Vectors
- Isolation of DNA from Mycobacterium tubercolosis
- Extraction of RNA from Intracellular Mycobacterium tuberculosis
- Transposon Mutagenesis in Mycobacteria Using Conditionally Replicating Mycobacteriophages
- Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB
- Gene Replacement using Pretreated DNA
- Gene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL as a Dominant Negative Selectable Marker
- Transcription Start-Site Mapping
- Fluorescence and Brightfield Cytology of Live M. tuberculosis Cells
- Phage Replication Technology for Diagnosis and Drug Susceptibility Testing
- Detection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization Strategy
- Restriction Fragment Length Polymorphism Typing of Mycobacteria
- Preparation of Culture Filtrate Proteins from Mycobacterium tuberculosis
- Analysis of the Capsule of Mycobacterium tuberculosis
- Analysis of the Lipids of Mycobacterium tuberculosis
- In Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosis
- 17 Macrophage Virulence Assays
- Analysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell Fractionation
- Real Time PCR Using Molecular Beacons
- Electronic Access to Mycobacterium tuberculosis Sequence Data
- Proteomics
- Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays
- Storage of Mycobacterial Strains
- Safety in the Laboratory