Angiotensin Protocols
The renin-angiotensin system has been in the spotlight for more than 100 years because of its vital role in blood pressure regulation, ion and fluid homeostasis, and tissue growth and remodeling. Recent advances in molecular genetic techniques have established that the renin-angiotensin system may w...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2001, 2001
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| Edition: | 1st ed. 2001 |
| Series: | Methods in Molecular Medicine
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- to The Renin-Angiotensin System
- Historical Perspective of the Renin –Angiotensin System
- Three Angiotensin Paradigms in One Patient
- Transgenic and Gene Targeting Methods
- Production and Use of Chimeric Mice
- Genetic Manipulation of the Renin-Angiotensin System Using Cre-loxP-Recombinase
- Systemic Delivery of aTransgene in Intact Animals by a Retroviral Vector
- Antisense Inhibition of the Renin-Angiotensin System
- Analysis of Transcriptional and Translational Mechanisms
- A nalysis of Transcriptional Control Mechanisms I
- Analysis of Transcriptional Control Mechanisms II Techniques for Characterization of trans-Acting Factors
- Analysis of Cytosolic Proteins that Bind to the 5? Leader Sequence of the Angiotensin AT1 Receptor by RNA Electromobility Shift Assay
- In Vitro Translation of the Angiotensin AT1 Receptor in Wheat-Germ Extracts
- Assays For Determination of Mrna Levels
- Quantitation of Angiotensin II Receptors by Competitive Reverse-Transcription Polymerase Chain Reaction
- Analysis of RNA by Northern-Blot Hybridization
- Use of the Ribonuclease Protection Assay for the Analysis and Characterization of Target mRNAs
- In Situ Hybridization
- Assays For Determination of Protein Contents and Enzyme Activity
- Immunohistochemical Detection of Angiotensin II Receptor
- Western Blotting
- Quantification of Angiotensin-Converting Enzyme (ACE) Activity
- Quantification of Angiotensin Peptides
- Radiolabeling of Angiotensin Peptides
- Assays for Radiolabel-Photoaffinity Labeling of Angiotensin Receptors
- Radioligand Binding Assay
- Autoradiographic Localization and Quantification of Components of the Renin-Angiotensin System in Tissues
- Biochemical Studies of The Renin-Angiotensin System
- Measurement of Intracellular Free Calcium Ion Concentration in Vascular Smooth Muscle Cells
- Measurement of Phospholipase D Activation in Vascular Smooth Muscle Cells
- Measurement of Cyclic AMP and Cyclic GMP in Xenopus Oocytes Stimulated with Angiotensin II and Atrial Natriuretic Factor
- Measurement of Nitric Oxide Formation
- Measuring Renin Secretion from Juxtaglomerular Cells
- Functional Studies of The Renin-Angiotensin System
- Measurement of Regional Blood Flow in the Kidney Using Laser-Doppler Flowmetry
- Measurement of Renal Tubular Angiotensin II
- The In Vitro Blood-Perfused Juxtamedullary Nephron Technique
- In Vitro Proximal Tubule Perfusion Preparation
- Isolated Resistance Artery Preparation
- Measurement of Vascular Density
- Functional Studies of the Cerebral Circulation