|
|
|
|
| LEADER |
04577nmm a2200301 u 4500 |
| 001 |
EB000285855 |
| 003 |
EBX01000000000000000050090 |
| 005 |
00000000000000.0 |
| 007 |
cr||||||||||||||||||||| |
| 008 |
090727 ||| eng |
| 020 |
|
|
|a 9781592591152
|
| 050 |
|
4 |
|a QD415-436
|
| 100 |
1 |
|
|a Lieberman, Benjamin A.
|e [editor]
|
| 245 |
0 |
0 |
|a Steroid Receptor Methods
|h Elektronische Ressource
|b Protocols and Assays
|c edited by Benjamin A. Lieberman
|
| 250 |
|
|
|a 1st ed. 2001
|
| 260 |
|
|
|a Totowa, NJ
|b Humana Press
|c 2001, 2001
|
| 300 |
|
|
|a XIV, 381 p
|b online resource
|
| 505 |
0 |
|
|a Protein Interaction Assays -- Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors -- Knockout Mice and Steroid Receptor Research -- Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors -- Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex -- Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System -- Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay -- Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcription In Vivo -- Cotransfection Assays and Steroid Receptor Biology -- Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System -- Cancer Research and Drug Discovery -- Solid Tumor Cancer Markers and Applications to Steroid Hormone Research --
|
| 505 |
0 |
|
|a Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay -- Combinatorial Chemistry in Steroid Receptor Drug Discovery -- Identification of Nuclear Receptor Interacting Proteins Using Yeast Two- Hybrid Technology
|
| 505 |
0 |
|
|a Bioinformatics -- Bioinformatics of Nuclear Receptors -- Phylogenetic Inference and Parsimony Analysis -- Purification Protocols -- Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems -- High-Yield Purification of Functionally Active Glucocorticoid Receptor -- Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor -- Large Scale Production of Nuclear Receptor Ligand-Binding Domains -- Physical Structure of Nuclear Receptor-DNA Complexes -- Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display -- Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes -- Steroid Hormone-Binding Assays -- Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors -- Steroid Hormone Metabolites and Hormone Binding Assays -- Vitamin D3 Analog Screening --
|
| 653 |
|
|
|a Biochemistry
|
| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
|
| 989 |
|
|
|b SPRPROT
|a Springer Protocols Archive 1981-2004
|
| 490 |
0 |
|
|a Methods in Molecular Biology
|
| 028 |
5 |
0 |
|a 10.1385/1592591159
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/1592591159?nosfx=y
|x Verlag
|3 Volltext
|
| 082 |
0 |
|
|a 572
|
| 520 |
|
|
|a Research into steroid receptor biology holds great promise for the treatment of cancer and endocrine disorders and for elucidating the fundamental biological mechanisms of gene expression. In Steroid Receptor Methods: Protocols and Assays, a distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA-binding characteristics and hormone-binding assays and with the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness. Finally, a discussion by example presents the most current bioinformatics tools and analytical techniques. Timely and highly accessible, Steroid Receptor Methods: Protocols and Assays provides biochemists and molecular biologists studying endocrine systems with a full range of the powerful tools needed for elucidating protein interactions with receptors and DNA, as well as for drug discovery
|