Atherosclerosis Experimental Methods and Protocols

Atherosclerosis-a major killer of people in Western societies-is not yet completely understood. In Atherosclerosis: Methods and Protocols, Angela Drew and a panel of experts have assembled a comprehensive collection of traditional and cutting-edge techniques for investigating this disease and its po...

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Bibliographic Details
Other Authors: Drew, Angela F. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2001, 2001
Edition:1st ed. 2001
Series:Methods in Molecular Medicine
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Animal Models of Diet-Induced Atherosclerosis
  • Mechanical Injury Models
  • Genetically Manipulated Models of Atherosclerosis in Mice
  • Lipoprotein Isolation and Analysis from Serum by Preparative Ultracentrifugation
  • Separation of Plasma Lipoproteins in Self-Generated Gradients of Iodixanol
  • Fractionation of Lipoprotein Subclasses in Self-Generated Gradients of Iodixanol
  • 7 Detecting Cholesteryl Ester Transfer Protein in Plasma
  • 8 Determination of Plasma Homocysteine
  • 9 Assay for Serum Glycated Lipoproteins
  • 10 Lipoprotein(a) Quantitation
  • Isolation and Characterization of Glycosaminoglycans from Human Atheromatous Vessels
  • Culture of Human Smooth Muscle Cells
  • Culture of Human Endothelial Cells
  • Assay for Cell Migration and Invasion of Vascular Smooth Muscle Cells
  • Collection and Processing of Arterial Specimens for Histological Analysis
  • Intravascular Ultrasound Imaging in the Quantitation of Atherosclerosis in Vivo
  • Identification of Cell Types and Quantification of Lesion Composition
  • 1Nonradioactive In Situ Hybridization in Atherosclerotic Tissue
  • Evaluation of Angiogenesis
  • Evaluation of Cell Proliferation in Human Atherosclerotic Lesions
  • Gene Transfer to the Vasculature Historical Perspective and Implications for Future Research Objectives
  • A Pig Model of Vein Graft Disease Applications for Potential Gene Therapies