Antigen Processing and Presentation Protocols
The processing and presentation of antigens by major histocompatibility complex (MHC) molecules is a crucial immunological phenomenon in infectious disease, malignancy, autoimmune disease, and transplantation. In Antigen Processing and Presentation Protocols, well-recognized and innovative experimen...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2001, 2001
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| Edition: | 1st ed. 2001 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Identification of CD8+T-Cell-Stimulating Antigen Genes in cDNA Libraries
- Generation of Antigen-Specific, LacZ-Inducible T-Cell Hybrids
- Photolabeling the Transporter Associated with Antigen Processing
- Application of One-Dimensional Isoelectric Focusing to Separate Different Major Histocompatibility Complex Class I Alleles and Determine Their Allelic Interactions with Transporter Associated with Antigen Processing (TAP)
- Analysis of Major Histocompatibility Complex Class I Interactions with Endoplasmic Reticulum Proteins
- Methods of Purification of Heat Shock Protein-Peptide Complexes for Use as Vaccines Against Cancers and Infectious Diseases
- Random Peptide Libraries
- Neural Network Method for Predicting Peptides that Bind Major Histocompatibility Complex Molecules
- Analysis of T-Cell Response Using Altered Peptide Ligands
- Assays of Thymic Selection Fetal Thymus Organ Culture and In Vitro Thymocyte Dulling Assay
- Protocol for Diversion of a CD4+Response to theT-Helper 2 Cell Pathway
- Quantitating Presentation of MHC Class I-Restricted Antigens
- Purification of 20S Proteasomes
- Use of Proteasome Inhibitors to Examine Processing of Antigens for Major Histocompatibility Complex Class I Presentation
- Tracing the Route Taken by Peptides and Major Histocompatibility Complex Class I Molecules in Presentation of Exogenous Antigens
- Functional Analysis of Antigen Processing and Major Histocompatibility Complex Class II-Restricted Presentation
- Quantitation of Major Histocompatibility Complex Antigen Endocytosis Using Capture ELISA
- In Vitro Generation of Functional Human and Murine Dendritic Cells
- Genetic Engineering of Dendritic Cells Using Retrovirus-Based Gene Transfer Techniques
- Use of Vaccinia Virus Expression Vectors to Investigate Antigen Processing and Presentation
- Sindbis Virus-Based Vectors for the Study of Class I Antigen Presentation In Vitro and In Vivo
- Precision Genotyping of Human Leukocyte Antigen-A, -B and -C Loci Via Direct DNA Sequencing