Vascular Disease Molecular Biology and Gene Transfer Protocols
In Vascular Disease: Molecular Biology and Gene Therapy Protocols, Andrew Baker and a noted panel of expert investigators describe today's most powerful molecular methods for investigating the pathogenesis of vascular disease. These detailed, easy-to-follow techniques range from methods that ha...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
1999, 1999
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| Edition: | 1st ed. 1999 |
| Series: | Methods in Molecular Medicine
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Identification of Novel Protein Kinases in Vascular Cells
- Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells
- Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes
- Use of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle Cells
- Nuclear Run-On Assay to Study Gene Transcription in Vascular Cells
- Electromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle Cells
- Molecular Analysis of MRNA Expression in Vascular Cells
- Measurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis
- Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases
- Localization of Gene Expression in Human Atherosclerotic Lesions by In SituHybridization
- RNase Protection Assays for Quantitation of Gene Expression in Vascular Tissue
- Genetics in Vascular Disease
- Detection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism Analysis
- Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis
- Radiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted Genes
- Isolation of Genes Expressed in Vasular Tissue
- Efficient Extraction of RNA from Vascular Tissue
- Preparation of cDNA Libraries from Vascular Cells
- Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells
- Cloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCR
- Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques
- The Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular Cells
- Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and Tissus
- Detection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick-End Labeling (TUNEL)
- In Vitro Detection of Apoptosis in Isolated Vascular Cells
- Molecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence Analysis
- In Vitro Methods to Express Foreign Genes in Vasular Cells
- Simple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular Cells
- Generation of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular Cells
- Hemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular Cells
- High-Efficiency and Low-Toxicity Adenovirus-Assisted Endothelial Transfection
- Gene Transfer in Vascular Cells Using an Engineered Na-H Exchanger (NHE1) as a Selectable Marker
- Use of Retroviruses to Express Exogenous Genes in Vascular Smooth Muscle Cells
- Embryonal Stem (ES) Cell-Derived Macrophages
- Delivery of Recombinant Adenoviruses to Human Saphenous Vein
- In Vivo Vascular Gene Transfer Protocols
- An In Vivo Angiogenesis Assay to Study Positive and Negative Regulators of Neovascularization
- Efficient Liposome-Mediated Gene Transfer to Rabbit Carotid Arteries In Vivo
- Gene Delivery to Rabbit Arteries Using the Collar Model
- Delivery of Antisense Oligonucleotides to the Vascular Wall
- Local Gene Delivery of Recombinant Adenoviruses to the Rat Carotid Artery In Vivo