Chemokine Protocols
In the past decade research has established the biological importance of chemokines: they play a major role in leukocyte trafficking, in the recruitment of leukocytes to inflammatory sites, and are coreceptors along with CD4 for HIV cell entry. In Chemokine Protocols, expert investigators describe i...
| Other Authors: | , , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2000, 2000
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| Edition: | 1st ed. 2000 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Purification of Chemokines from Natural Sources
- Cloning of Novel Chemokines Using a Signal Sequence Trap Method
- Chemokine Expression in Insect Cells
- Expression of Chemokines in Escherichia coli
- Expression of Chemokines in the Periplasmic Space of E. coli
- Synthesis of Chemokines
- Identification of Novel Chemokines From Expressed Sequence Tag Databases
- Purification of Recombinant Chemokines from E. coli
- Chemokine Receptor Cloning
- Generation of Stable Cell Lines Expressing Chemokine Receptors
- Modified Microchemotaxis Assays
- Transwell Chemotaxis
- Endothelial Cell Chemotaxis Assays
- Radiolabeled Chemokine Binding Assays
- Scintillation Proximity Binding Assay
- Calcium Mobilization
- Actin Polymerization
- Reactive Oxygen Release
- Histamine Release
- Measurement of Phosphoinositide 3-Kinase Activity
- Glycosaminoglycan Binding Assays
- CFU-A Assay for Measurement of the Antiproliferative Effects of Chemokines on Murine Early Hemopoietic Progenitors
- Downmodulation and Recycling of Chemokine Receptors
- Analysis of Chemokine Receptor Endocytosis and Recycling
- Chemokine Inhibition of HIV Infection
- 26The Production of Chemokine Specific Monoclonal Antibodies
- Monoclonal Antibodies to Chemokine Receptors
- Targeted Expression of Chemokines In vivo
- Chemokine Knockout Mice
- Chemokine Receptor Knockout Mice
- Measurement of Eosinophil Accumulation In vivo
- Murine Model of Allergic Lung Inflammation
- Murine Models of Airway Inflammation
- Rat Models of Respiratory Inflammation
- Murine Model of Crescentic Nephritis
- Rabbit Models of Pneumonia, Peritoneal Sepsis, and Lung Injury