Calcium-Binding Protein Protocols Volume 2: Methods and Techniques
Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols- Volume 1: Reviews and Case Historie...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2002, 2002
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| Edition: | 1st ed. 2002 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Methods and Techniques to Study
- Quantitative Analysis of Ca2+-Binding by Flow Dialysis
- Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators
- Deconvolution of Calcium-Binding Curves
- Absorption and Circular Dichroism Spectroscopy
- Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins
- Steady-State Fluorescence Spectroscopy
- Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins
- Surface Plasmon Resonance of Calcium-Binding Proteins
- Differential Scanning Calorimetry
- Isothermal Titration Calorimetry
- Multiangle Laser Light Scattering and Sedimentation Equilibrium
- Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in their Interactions with Regulatory Targets
- Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry
- Synthetic Calcium-Binding Peptides
- Proteolytic Fragments of Calcium-Binding Proteins
- Electron Magnetic Resonance Studies of Calcium-Binding Proteins
- Cadmium-113 and Lead-207 NMR Spectroscopic Studies of Calcium-Binding Proteins
- Calcium-43 NMR of Calcium-Binding Proteins
- Exploring Familial Relationships Using Multiple Sequence Alignment
- Structure Determination by NMR
- Protein Structure Calculation from NMR Data
- Shape and Dynamics of a Calcium-Binding Protein Investigated by Nitrogen-15 NMR Relaxation
- The Use of Dipolar Couplings for the Structure Refinement of a Pair of Calcium-Binding EGF Domains
- Vector Geometry Mapping
- Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography
- Enzymatic Assays to Compare Calmodulin Isoforms, Mutants, and Chimeras
- Gene Expression inTransfected Cells
- Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins
- Studying the Spatial Distribution of Ca2+-Binding Proteins