Nuclease Methods and Protocols
Nucleases, the enzymes that cleave and shape nucleic acids, are key regulators of cellular metabolism. Angiogenesis and the unfolded protein response are just a few instances of important intra- and extracellular pathways regulated by nucleases. In Nuclease Methods and Protocols, researchers at the...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2001, 2001
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| Edition: | 1st ed. 2001 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Relating Nucleas Structure and Function
- Crystallization and Crystal Structure Determination of Ribonuclease A-Ribonuclease Inhibitor Protein Complex
- Methods for Studying the Interaction of Barnase with Its Inhibitor Barstar
- Assaying In Vitro Refolding of RNases by Mass Spectrometry
- Dissecting Nucleases into Their Structural and Functional Domains
- Using Electrostatics to Define the Active Site of Serratia Endonuclease
- Homology Modeling and Simulations of Nuclease Structures
- Methods for Determining Activity and Specificity of DNA Binding and DNA Cleavage by Class II Restriction Endonucleases
- Engineered Properties and Assays for Human DNase I Mutants
- Nucleases in the Clinic
- Assays for Human DNase I Activity in Biological Matrices
- Evaluation of Retroviral Ribonuclease H Activity
- Assays for Detection of RNase A Superfamily Ribonucleases
- Assay for Antitumor and Lectin Activity in RNase Homologs
- Isolation and Enzymatic Activity of Angiogenin
- Characterizing Nuclease Activity
- Processivity of DNA Repair Enzymes
- Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction of Pancreatic-Type Ribonucleases
- Ier1 p: A Kinase and Site-Specific Endoribonuclease
- Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleases
- Quantitating mRNAs with Relative and Competitive RT-PCR
- Expressing Self-Incompatibility RNases (S-RNases) in Transgenic Plants
- Molecular Cloning, Tissue Distribution, and Chromosomal Localization of the Human Homolog of the R2/Th/Stylar Ribonuclease Gene Family
- Inhibitors and Activators of Nucleases
- Ribonuclease Inhibitors
- Producing Soluble Recombinant RNases and Assays to Measure Their Interaction with Interferon-? In Vitro?
- Assays for the Evaluation of HIV-1 Integrase Inhibitors
- The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides
- The 2–5A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI)
- Preparation and Preclinical Characterization of RNase-Based Immunofusion Proteins
- Assays Using Nucleases
- Restriction Endonucleases and Their Uses
- Isolation and Characterization of an Unknown Restriction Endonuclease
- Gene Modification with Hapaxoterministic Restriction Enzymes
- Techniques to Measure Nucleic Acid-Protein Binding and Specificity
- Analyzing the Developmental Expression of Sigma Factors with S1-Nuclease Mapping
- Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays